Workshop #2: SAMPREP (Sample Attributes for Multiple-techniques and Principal Requirements for Experiments in Pan-structural biology) Workshop
| Date/Time |
Friday, July 7, 2023 @ 8:30 AM |
|
|
Registration Fees |
| Location |
Baltimore Marriott Waterfront | Room TBD
|
|
|
Regular: $125 Per Person
Retired: $125 Per Person
PostDoc: $75 Per Person
Student: $50 Per Person
Corporate: $125 Per Person
|
| Max Capacity |
NA |
|
|
|
| Main Contact |
Christina Zimanyi
Silvia Russi
|
|
|
|
Description
| |
SPONSORED BY: |
| |
 |
The routine use of multi-technique approaches to answer the most complex and challenging questions in structural biology has dramatically increased in recent years. We believe the 2023 meeting in Baltimore is an excellent destination for bringing together Cryo-EM, MX and SAS experts to prepare students and investigators new to the methods for the successful application of these techniques. We are particularly excited to offer this workshop in conjunction with the stated goal of the 2023 meeting organizing committee to attract early career scientists via a more education focused meeting program. This one-day workshop will provide introduction to both the theory of the methods and best practices common to the field with a focus on the importance of the quality of samples. In addition to synchrotron sources, this workshop will devote content to laboratory best practices in the preparation of samples and their manipulation at the home lab and their transportation to Cryo-EM centers and synchrotrons. In contrast to the more intensive courses at synchrotrons around the world that extend across several days, but usually cover only one technique, this single day of carefully constructed lectures and tutorials is an introduction aimed at improving the chances for success in preliminary experiments. The material is appropriate for investigators new to the field, or for those with experience in one structural biology technique to gain the knowledge they need to pursue others, and elevate the standards for published data and peer-review. The workshop format will include lectures and a selection of hands-on practical exercises. Throughout the workshop the emphasis will be on practical application: knowing how to judge sample quality, how to troubleshoot during sample preparation, and the expectations for a successful experiment and acceptable publication.
Schedule*
| Time | Instructor/Topic |
|---|
| 8:30 AM ET |
Introduction
Cross section between Solution Scattering (SAS), macromolecular X-ray crystallography (MX), & cryo-electron microscopy (Cryo-EM)
Speakers: TBA
|
| 9:00 AM ET |
Sample Quality
The importance of purification and preparation (aggregation, buffer selection, etc)
Speakers: James Byrnes & Marco Mazzorana
|
| 10:00 AM ET |
Coffee Break
|
| 10:15 AM ET |
Biochemical and Biophysical Characterization and Small Angle X-ray Scattering
Speakers: Kushol Gupta & Thomas Weiss
|
| 11:15 AM ET |
Stretch Break |
| 11:25 AM ET |
CryoEM
How much does the quality of cryo-EM data depend on your sample?
Speakers: Christina Zimanyi & Liguo Wang
|
| 12:25 PM ET |
Working Lunch with 2 short talks from vendors |
| 1:30 PM ET |
The several facets of Crystallization
From large to nanosized crystals
Speakers: Sarah Bowman & Vivian Stojanof
|
| 2:30 PM ET |
Sample Transportation
Preparing your samples for safe arrival at Centers and Facilities – Tips and Tricks
Speakers: Silvia Russi & James Byrnes & Christina Zimanyi
|
| 3:15 PM ET |
Coffee Break
|
| 3:30 PM ET |
Integrated Structural Biology : Case studies
Invited speaker TBA
|
| 4:15 PM ET |
Introduction to panel discussion.
|
| 4:30 PM ET |
Panel Discussion
Share your stories (all participants). Participants can also share protocols or data for feedback from the panel of experts.
All speakers
|
| 5:30 PM ET |
Conclusion
|
*Tentative and subject to change.
Instructors
 |
|
 |
|
 |
|
Sarah EJ Bowman
National High-Throughput Crystallization Center, Hauptman-Woodward Medical Research Institute
|
|
James Byrnes
Center for Biomolecular Structure, NSLS II, Brookhaven National Laboratory
|
|
Kushol Gupta
Perelman School of Medicine, University of Pennsylvania & Johnson Foundation Structural Biology and Biophysics Core
|
| |
|
|
|
|
| |
|
 |
|
|
|
Marco Mazzorana
Diamond Light Source, Ltd (UK)
|
|
Silvia Russi
Structural Macromolecular Biology Division, SSRL, SLAC National Accelerator Laboratory
|
|
Vivian Stojanoff
Center for Biomolecular Structure, NSLS II, Brookhaven National Laboratory
|
| |
|
|
|
|
|
|
|
|
|
|
|
Thomas Weiss
Structural Macromolecular Biology Division, SSRL, SLAC National Accelerator Laboratory
|
|
Christina Zimanyi
National Center for CryoEM Access and
Training, New York Structural Biology Center
|
|
|
|
Diamond Level Sponsors [$5,000+]
