Workshop #2: SAMPREP (Sample Attributes for Multiple-techniques and Principal Requirements for Experiments in Pan-structural biology) Workshop

Date/Time Friday, July 7, 2023 @ 8:30 AM     Registration Fees
Location

Kent AB

   

Regular: $125 Per Person

Retired: $125 Per Person

PostDoc: $75 Per Person

Student: $50 Per Person

Corporate: $125 Per Person

Max Capacity NA      
Main Contact

Christina Zimanyi

Silvia Russi

     

 

Description

  SPONSORED BY:
 
 
 

 
The routine use of multi-technique approaches to answer the most complex and challenging questions in structural biology has dramatically increased in recent years. We believe the 2023 meeting in Baltimore is an excellent destination for bringing together Cryo-EM, MX and SAS experts to prepare students and investigators new to the methods for the successful application of these techniques. We are particularly excited to offer this workshop in conjunction with the stated goal of the 2023 meeting organizing committee to attract early career scientists via a more education focused meeting program. This one-day workshop will provide introduction to both the theory of the methods and best practices common to the field with a focus on the importance of the quality of samples. In addition to synchrotron sources, this workshop will devote content to laboratory best practices in the preparation of samples and their manipulation at the home lab and their transportation to Cryo-EM centers and synchrotrons. In contrast to the more intensive courses at synchrotrons around the world that extend across several days, but usually cover only one technique, this single day of carefully constructed lectures and tutorials is an introduction aimed at improving the chances for success in preliminary experiments. The material is appropriate for investigators new to the field, or for those with experience in one structural biology technique to gain the knowledge they need to pursue others, and elevate the standards for published data and peer-review. The workshop format will include lectures and a selection of hands-on practical exercises. Throughout the workshop the emphasis will be on practical application: knowing how to judge sample quality, how to troubleshoot during sample preparation, and the expectations for a successful experiment and acceptable publication.
 

Schedule*

TimeInstructor/Topic
8:30 AM ET

Introduction
Cross section between Solution Scattering (SAS), macromolecular X-ray crystallography (MX), & cryo-electron microscopy (Cryo-EM)
Speakers: Christina Zimanyi, Silvia Russi, Thomas Weiss

8:45 AM ET

Sample Quality
The importance of purification and preparation
Speakers: James Byrnes & Marco Mazzorana

9:45 AM ET

Coffee Break

10:00 AM ET

Biochemical and Biophysical Characterization and Small Angle X-ray Scattering
Speakers: Kushol Gupta & Thomas Weiss

11:00 AM ET CryoEM
How much does the quality of cryo-EM data depend on your sample?
Speakers: Christina Zimanyi & Liguo Wang
12:00 PM ET

Lunch with a 15 minute talk from Refeyn on Mass Photometry, then free time

1:15 PM ET The several facets of Crystallization
From large to nanosized crystals
Speakers: Sarah Bowman & Vivian Stojanof
2:15 PM ET

Sample Transportation
Preparing your samples for safe arrival at Centers and Facilities – Tips and Tricks
Speakers: Silvia Russi, Sarah Bowman, James Byrnes & Christina Zimanyi

3:00 PM ET

Coffee Break

3:15 PM ET

Integrated Structural Biology: A Case study

Combining SAXS and cryo-EM to study dynamic protein systems: a case study with cobalamin-dependent methionine synthase

Invited speaker: Max Watkins

4:15 PM ET

Panel Discussion
Share your stories (all participants). Participants can also share protocols or data for feedback from the panel of experts.
All speakers.

5:30 PM ET

Conclusion

 *Tentative and subject to change.

 

Instructors

         

Sarah EJ Bowman

National High-Throughput Crystallization Center, Hauptman-Woodward Medical Research Institute

 

James Byrnes

Center for Biomolecular Structure, NSLS II, Brookhaven National Laboratory

 

Kushol Gupta

Perelman School of Medicine, University of Pennsylvania & Johnson Foundation Structural Biology and Biophysics Core

         
         

Marco Mazzorana

Diamond Light Source, Ltd (UK) 

 

Silvia Russi

Structural Macromolecular Biology Division, SSRL, SLAC National Accelerator Laboratory

 

Vivian Stojanoff

Center for Biomolecular Structure, NSLS II, Brookhaven National Laboratory

         

     

Thomas Weiss

Structural Macromolecular Biology Division, SSRL, SLAC National Accelerator Laboratory

 

Christina Zimanyi

National Center for CryoEM Access and
Training, New York Structural Biology Center