Workshop 5: CryoEM sample preparation training using center merit badges

Date/Time: Friday, July 29, 2022 @ 9:00 AM PT
Location: OFFSITE!  Workshop held off site at PNCC which is a 15 min walk or 10 min Tri-Met trip from the Portland Marriott Downtown Waterfront, 1401 SW Naito Pkwy, Portland, OR 97201
Organizational Contact: Ed Eng

 


Description

Cryo-electron microscopy (cryoEM) is a method used to determine high-resolution, three-dimensional structures of samples that could not be used with other techniques in the past, such as samples containing mixtures of different biological structures or intact cells. CryoEM instrumentation is being installed in many research institutions and there is a need to learn the best practices available in the field. This workshop, which will be held on Friday, July 29, 2022, will focus on cryoEM educational resources available to researchers and to provide hands-on training with cryoEM sample preparation instrumentation.

The objective is to learn how to interface with the NIH sponsored Transformative High-Resolution CryoEM Program, which offers a comprehensive online cryoEM curriculum that familiarizes new users with cryoEM equipment and workflow. Lectures and practicals will focus on everything from how to use the national cryoEM service centers, media-rich curriculum to augment users’ own hands-on training, and earning cryoEM merit badges. CryoEM proficiency badges are awarded to users in three main skill areas (Sample preparation, Microscope operations and Data processing). This workshop, in particular, will focus on the “Sample preparation” merit badges. After trainees complete a necessary set of requirements, they may apply for a merit badge. Receipt of a merit badge will qualify the user for independence, and be cross-honored at other national service centers for instrumentation use. This workshop will benefit those who want to use cryoEM centers, or who may be new to cryoEM workflows. This workshop will be held offsite at PNCC/OHSU where staff and students must provide proof of full COVID-19 immunization. Admission into this workshop is considered conditional until proof of full COVID-19 vaccination is provided or a medical or religious exception is approved by OHSU.

 

Instructors

Michael Cianfrocco
University of Michigan

Craig Yoshioka
PNCC/OHSU

Claudia López
PNCC/OHSU

Michael Schmid
S2C2/Stanford

       

Peter Shen
University of Utah

Ed Eng
NCCAT/NYSBC

Wen Jiang
Purdue University

Yingjie Victor Chen
Purdue University

 

Schedule 

This workshop will be part of the main program of the ACA meeting, but held offsite at PNCC where the cryoEM instrumentation and laboratory space will be available. Due to OHSU's COVID-19 Immunizations and Education policy, everyone visiting OHSU will need to provide proof of full COVID-19 vaccination or attestation from their home institution that they are compliant with OHSU and Oregon Health Authority vaccination policies (https://news.ohsu.edu/2021/08/04/ohsu-covid-19-vaccination-requirement-update) .  For those with medical or religious exception approvals, a letter from their home institutions will be required. The registration cap will be 40 users. Registrants will be asked to rank the practical station offerings and be able to attend up to 3 stations with the intention of earning NIH cryoEM center merit badges. However, certain merit badge stations may require the full day to earn based on the registrants starting level.

 Start EndInstructor/Topic 
9:00  9:05  Craig Yoshioka, OHSU & Ed Eng, NYSBC – Welcome and introduction to NIH Transformative High-Resolution CryoEM Program
9:05 10:00 CryoEM Curriculum Development Part I (Moderated by Michael Schmid, S2C2)
    (9:05-9:30) Peter Shen, University of Utah – cryoEM101: How to use media and data visualization to aid the training efforts of newcomers to the field
    (9:35-10:00) Wen Jiang & Yingjie Victor Chen, Purdue University – cryoVR: How Virtual Reality can be used to familiarize users with cryoEM equipment
10:00 10:15 Coffee break
10:15 12:30 cryoEM Merit badges Part I
    (10:15-10:30) Ed Eng, NYSBC & Claudia López, OHSU – Introduction to cryoEM Merit Badges and practical stations
   

(10:30-12:00)

Students at their 1st practical stations (must pre-register and rank top 3 choices)
1) Station A: cryoVR: Virtual Reality augmented cryoEM training
2) Station B: Plunge freezing with the Vitrobot Mark IV
3) Station C: Plunge freezing with the Leica EM GP
4) Station D: Autoloaders: Autogrid clipping and loading
5) Station E: Shipping: cryoEM pucks and dry shipping
6) Station F: Negative stain
7) Station G: Support films and grid treatments
8) Station H: VitroJet demonstration for blot free vitrification

12:00 12:45 Lunch and discussion
12:45 1:30 CryoEM Curriculum Development Part II 
    (12:45-1:10) Michael Cianfrocco, University of Michigan – cryoEDU: How to learn and practice cryoEM processing with limited computational resources
    (1:10-1:30) Roundtable and general Q&A (Moderated by Michael Schmid, S2C2 and Claudia López, OHSU) 
1:30 4:30

cryoEM Merit badges Part II
Students rotate through 2nd and 3rd practical stations (must pre-register and rank top 3 choices)
1) Station A: cryoVR: Virtual Reality augmented cryoEM training
2) Station B: Plunge freezing with the Vitrobot Mark IV
3) Station C: Plunge freezing with the Leica EM GP
4) Station D: Autoloaders: Autogrid clipping and loading
5) Station E: Shipping: cryoEM pucks and dry shipping
6) Station F: Negative stain
7) Station G: Support films and grid treatments
8) Station H: VitroJet demonstration for blot free vitrification

4:30  

Wrap up and merit badge certification awards

4:30 6:00

Coffee and networking