Workshop 4: Hands-on Single-Particle Cryo-EM Data Analysis with cryoEDU

Date/Time: Friday, July 29, 2022 @ 9:00 AM PT
Location:
ROOM TBA 
Portland Marriott Downtown, Portland, Oregon
Organizational Contact: Michael Cianfrocco, Ph.D.

 


    

        

Description

Single-particle cryo-electron microscopy (cryo-EM) is rapidly becoming the method of choice for structure determination of dynamic proteins, integral membrane protein complexes, large macromolecular assemblies, and multi-protein complexes. This powerful technique is now capable of resolving biological specimens to better than 2 Å resolution and has been used to solve high-resolution structures of specimens as small as ~50 kilodaltons. Cryo-EM instrumentation is being installed in many universities across the globe and is also available at large multi-user facilities both in the U.S. and worldwide. However, there are many practical aspects of cryo-EM that are not covered in literature nor in research talks. We believe that this one-day workshop will cover many of the salient practical issues new cryo-EM users need to consider in order to bring a project from purified sample to completed atomic model.

This workshop, which will be held on Friday, July 29th 2022, will introduce structural biologists to hands-on cryo-EM data processing. Our workshop will utilize a cryo-EM data simulator with cryoEDU to enable real time feedback on how data processing decisions affect downstream outcomes. cryoEDU is a newly funded NIH cryo-EM/ET curriculum development grant to educate cryo-EM practitioners on how to analyze and interpret cryo-EM data. Lectures will focus on the steps involved in producing a cryo-EM map, from specimen preparation and data collection through to image processing, reconstruction, modeling, and validation.

 

Instructors

Michael Cianfrocco, Ph.D.
Assistant Professor
Department of Biological Chemistry
Research Assistant Professor
Life Sciences Institute
University of Michigan
 

Elizabeth Kellogg, Ph.D.

Assistant Professor
Department of Molecular Biology and Genetics
Cornell University

Mark Herzik, Ph.D.

Assistant Professor
Department of Chemistry and Biochemistry
UC San Diego

Melody Campell, Ph.D.

Assistant Professor
Basic Sciences Division
Fred Hutchinson Cancer Research Center

 

Schedule 

 Start EndInstructor/Topic 
9:00  9:15  Michael Cianfrocco, Ph.D  - Welcome & introduction
9:15 10:00 Melody Campbell, Ph.D - Practical introduction to single particle analysis
10:00 10:45 Liz Kellogg, Ph.D - Preprocessing cryo-EM datasets
10:45 11:00 Break
11:00  12:00 Michael Cianfrocco, Ph.D - Preprocessing with data simulator (hands on)
12:00 2:00

Lunch break:
● Joint lunch with cryo-ET workshop
● Free time to use the cryo-EM simulator.
● Visit sponsor tables

2:00 2:45

Mark Herzik, Ph.D - 2D classification & 3D analysis

2:45 3:30

Michael Cianfrocco - Data simulator 2D analysis (hands on)

3:30 3:45

Break

3:45 4:30 

Mark Herzik, Ph.D- 3D classification and 3D refinement (hands on)

4:30 6:30

Joint reception with cryo-ET workshop and cryo-EM SIG with Liz
Kellogg, Ph.D